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Koi's oxygen requirement is generally believed to be a concentration of around 6 parts per million (PPM) of oxygen in the water.

In 1959, A. Krogh, in his "The Comparative Physiology of Respiratory Mechanisms", showed that a 200 gram Koi requires 100 milliliters of oxygen per kilogram per hour. His tests on a 70,000 gram man showed we require 200 milliliters of oxygen per kilogram per hour at rest, which jumps to 4,000 ml/kg/hr at maximum work levels.

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